| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ICOS (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanati |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ICOS |
| Gene ID: | 29851 |
| Uniprot ID: | ICOS_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_036224.1 |
| Immunogen Sequence: | KGGQILCDLTKTKGS |
| Post Translational Modifications | N-glycosylated. |
| Function | Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. |
| Protein Name | Inducible T-Cell Costimulator Activation-Inducible Lymphocyte Immunomediatory Molecule Cd Antigen Cd278 |
| Database Links | Reactome: R-HSA-1257604 Q9Y6W8-1 Reactome: R-HSA-2219530 Q9Y6W8-1 Reactome: R-HSA-388841 Q9Y6W8-1 Reactome: R-HSA-6811558 Q9Y6W8-1 Reactome: R-HSA-9725371 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Secreted |
| Alternative Antibody Names | Anti-Inducible T-Cell Costimulator antibody Anti-Activation-Inducible Lymphocyte Immunomediatory Molecule antibody Anti-Cd Antigen Cd278 antibody Anti-ICOS antibody Anti-AILIM antibody |
Information sourced from Uniprot.org