• Western blot analysis of extracts of various cell lines, using ICAM4 antibody (STJ29574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ICAM4 antibody (20-140) (STJ29574)

SKU:
STJ29574

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ICAM4 (20-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ICAM4
Gene ID: 3386
Uniprot ID: ICAM4_HUMAN
Immunogen Region: 20-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human ICAM4 (NP_001034221.1).
Immunogen Sequence: VGSALGRRTKRAQSPKGSPL APSGTSVPFWVRMSPEFVAV QPGKSVQLNCSNSCPQPQNS SLRTPLRQGKTLRGPGWVSY QLLDVRAWSSLAHCLVTCAG KTRWATSRITAYSVPGGLLG G
Tissue Specificity Erythrocytes.
Post Translational Modifications N- and O-glycosylated.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
Protein Name Intercellular Adhesion Molecule 4
Icam-4
Landsteiner-Wiener Blood Group Glycoprotein
Lw Blood Group Protein
Cd Antigen Cd242
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-216083
Cellular Localisation Isoform Long: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Short: Secreted
Cell Membrane
Alternative Antibody Names Anti-Intercellular Adhesion Molecule 4 antibody
Anti-Icam-4 antibody
Anti-Landsteiner-Wiener Blood Group Glycoprotein antibody
Anti-Lw Blood Group Protein antibody
Anti-Cd Antigen Cd242 antibody
Anti-ICAM4 antibody
Anti-LW antibody

Information sourced from Uniprot.org

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