• Immunofluorescence analysis of paraffin-embedded Rat lung tissue using Icam1 Rabbit polyclonal antibody (STJ11105185) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded Mouse lung tissue using Icam1 Rabbit polyclonal antibody (STJ11105185) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates, using Icam1 Rabbit polyclonal antibody (STJ11105185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Icam1 antibody (28-493 aa) (STJ11105185)

SKU:
STJ11105185

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Icam1
Gene ID: 25464
Uniprot ID: ICAM1_RAT
Immunogen Region: 28-493 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 28-493 of rat Icam1. (NP_037099.1).
Immunogen Sequence: QVSIHPTEAFLPRGGSVQVN CSSSCEDENLGLGLETNWMK DELSSGHNWKLFKLSDIGED SRPLCFENCGTTQSSASATI TVYSFPERVELDPLPAWQQV GKNLILRCLVEGGAPRTQLS VVLLRGNETLSRQAVDGDPK EITFTVLASRGDHGANFSCF TELDLRPQGLSLFKNVSEVR QLRTFDLPTRVLKLDTPDLL EVGTQQKFLCSLEGLFPASE AQIYLEMGGQMLTLESTNS
Post Translational Modifications Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Cd Antigen Cd54
Database Links Reactome: R-RNO-198933
Reactome: -RNO-216083
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Intercellular Adhesion Molecule 1 antibody
Anti-Icam-1 antibody
Anti-Cd Antigen Cd54 antibody
Anti-Icam1 antibody
Anti-Icam-1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance