Western blot analysis of Raji, using ICAM-1/CD54 antibody (STJ11103840) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ICAM1 antibody (250-480) (STJ11103840)

SKU:
STJ11103840
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ICAM1
Gene ID: 3383
Uniprot ID: ICAM1_HUMAN
Immunogen Region: 250-480
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM-1/CD54 (NP_000192.2).
Immunogen Sequence: VHLALGDQRLNPTVTYGNDS FSAKASVSVTAEDEGTQRLT CAVILGNQSQETLQTVTIYS FPAPNVILTKPEVSEGTEVT VKCEAHPRAKVTLNGVPAQP LGPRAQLLLKATPEDNGRSF SCSATLEVAGQLIHKNQTRE LRVLYGPRLDERDCPGNWTW PENSQQTPMCQAWGNPLPEL KCLKDGTFPLPIGESVTVTR DLEGTYLCRARSTQGEVTRK VTVNVLSPRYE
Post Translational Modifications Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Major Group Rhinovirus Receptor
Cd Antigen Cd54
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-216083
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-877300
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Intercellular Adhesion Molecule 1 antibody
Anti-Icam-1 antibody
Anti-Major Group Rhinovirus Receptor antibody
Anti-Cd Antigen Cd54 antibody
Anti-ICAM1 antibody

Information sourced from Uniprot.org

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