• Anti-ICAM1 antibody (120-320) (STJ11103325)
  • Anti-ICAM1 antibody (120-320) (STJ11103325)
  • Immunohistochemistry analysis of paraffin-embedded rat lung using ICAM1 Rabbit polyclonal antibody (STJ11103325) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using ICAM1 Rabbit polyclonal antibody (STJ11103325) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat lung, using ICAM1 antibody (STJ11103325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ICAM1 antibody (120-320) (STJ11103325)

SKU:
STJ11103325

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ICAM1 (120-320) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Icam1
Gene ID: 15894
Uniprot ID: ICAM1_MOUSE
Immunogen Region: 120-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of mouse ICAM1 (NP_034623.1).
Immunogen Sequence: LPAWQQVGKDLTLRCHVDGG APRTQLSAVLLRGEEILSRQ PVGGHPKDPKEITFTVLASR GDHGANFSCRTELDLRPQGL ALFSNVSEARSLRTFDLPAT IPKLDTPDLLEVGTQQKLFC SLEGLFPASEARIYLELGGQ MPTQESTNSSDSVSATALVE VTEEFDRTLPLRCVLELADQ ILETQRTLTVYNFSAPVLTL S
Tissue Specificity Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.
Post Translational Modifications Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Mala-2
Myd10
Cd Antigen Cd54
Database Links Reactome: R-MMU-198933
Reactome: -MMU-216083
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Intercellular Adhesion Molecule 1 antibody
Anti-Icam-1 antibody
Anti-Mala-2 antibody
Anti-Myd10 antibody
Anti-Cd Antigen Cd54 antibody
Anti-Icam1 antibody
Anti-Icam-1 antibody

Information sourced from Uniprot.org

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