• Western blot analysis of extracts of various cell lines, using HYI antibody (STJ114672) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-HYI antibody (80-260) (STJ114672)

SKU:
STJ114672

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HYI (80-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HYI
Gene ID: 81888
Uniprot ID: HYI_HUMAN
Immunogen Region: 80-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-260 of human HYI (NP_001177809.1).
Immunogen Sequence: PGRQAAFREGLEQAVRYAKA LGCPRIHLMAGRVPQGADRI AVKAEMEAVFLENLRHAAGV LAQEDLVGLLEPINTRITDP QYFLDTPQQAAAILQKVGRP NLQLQMDIFHWQIMDGNLTG NIREFLPIVGHVQVAQVPGR GEPSSPGELNFPYLFQLLED EGYKGFVGCEYQPRGDTVEG L
Function Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
Protein Name Putative Hydroxypyruvate Isomerase
Endothelial Cell Apoptosis Protein E-Ce1
Alternative Antibody Names Anti-Putative Hydroxypyruvate Isomerase antibody
Anti-Endothelial Cell Apoptosis Protein E-Ce1 antibody
Anti-HYI antibody
Anti-HT036 antibody
Anti-SB156 antibody

Information sourced from Uniprot.org

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