Immunofluorescence analysis of Neuro-2a cells using HTR2A Rabbit polyclonal antibody (STJ11103369) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HTR2A antibody (1-100) (STJ11103369)

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STJ11103369
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Short Description :Rabbit polyclonal HTR2A (1-100) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IF/ICC/ELISA
Host:Rabbit
Reactivity:Human/Mouse
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:HTR2A
Gene ID:3356
Uniprot ID:5HT2A_HUMAN
Immunogen Region:1-100
Immunogen Sequence:MDILCEENTSLSSTTNSLMQ LNDDTRLYSNDFNSGEANTS DAFNWTVDSENRTNLSCEGC LSPSCLSLLHLQEKNWSALL TAVVIILTIAGNILVIMAVS
Specificity:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HTR2A (NP_000612.1).
Tissue Specificity Detected in brain cortex (at protein level). Detected in blood platelets.
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. (Microbial infection) Acts as a receptor for human JC polyomavirus/JCPyV.
Protein Name 5-Hydroxytryptamine Receptor 2a
5-Ht-2
5-Ht-2a
Serotonin Receptor 2a
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Dendrite
Axon
Cytoplasmic Vesicle
Membrane
Caveola
Presynapse
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 2a antibody
Anti-5-Ht-2 antibody
Anti-5-Ht-2a antibody
Anti-Serotonin Receptor 2a antibody
Anti-HTR2A antibody
Anti-HTR2 antibody

Information sourced from Uniprot.org

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