Anti-HSPG2 antibody (STJA0007971)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human HSPG2
General Information
| Short Description | Rabbit polyclonal anti-Basement membrane-specific heparan sulfate proteoglycan core protein for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HSPG2 |
| Gene ID | 3339 |
| Uniprot ID | PGBM_HUMAN |
| Immunogen | Synthesized peptide derived from human HSPG2 |
| Specificity | This antibody detects endogenous levels of HSPG2 at Human, Mouse |
Additional Info
| Post Translational Modifications | Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide. O-glycosylated with core 1 or possibly core 8 glycans. Contains three heparan sulfate chains. Also contains chondroitin sulfate. |
| Function | Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Endorepellin: Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6. LG3 peptide: Has anti-angiogenic properties that require binding of calcium ions for full activity. |
| Protein Name | Basement Membrane-Specific Heparan Sulfate Proteoglycan Core ProteinHspgPerlecanPlc Cleaved Into - Endorepellin - Lg3 Peptide |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-1971475Reactome: R-HSA-2022928Reactome: R-HSA-2024096Reactome: R-HSA-216083Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3656237Reactome: R-HSA-3656253Reactome: R-HSA-4420332Reactome: R-HSA-9694614Reactome: R-HSA-975634Reactome: R-HSA-977225Reactome: R-HSA-9820960Reactome: R-HSA-9833110Reactome: R-HSA-9856532Reactome: R-HSA-9913351 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement Membrane |
| Alternative Antibody Names | Anti-Basement Membrane-Specific Heparan Sulfate Proteoglycan Core Protein antibodyAnti-Hspg antibodyAnti-Perlecan antibodyAnti-Plc Cleaved Into - Endorepellin - Lg3 Peptide antibodyAnti-HSPG2 antibody |
Information sourced from Uniprot.org