• Western blot analysis of extracts of various cell lines, using HSPA12A Rabbit monoclonal antibody (STJ11103163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HSPA12A antibody (50-150) [S3MR] (STJ11103163)

SKU:
STJ11103163

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA12A
Gene ID: 259217
Uniprot ID: HS12A_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HSPA12A (O43301).
Immunogen Sequence: SEQQSFLVVVAVDFGTTSSG YAYSFTKEPECIHVMRRWEG GDPGVSNQKTPTTILLTPER KFHSFGYAARDFYHDLDPNE AKQWLYLEKFKMKLHTTGDL T
Tissue Specificity Widely expressed with highest levels in brain, kidney and muscle.
Function Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.
Protein Name Heat Shock 70 Kda Protein 12a
Heat Shock Protein Family A Member 12a
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 12a antibody
Anti-Heat Shock Protein Family A Member 12a antibody
Anti-HSPA12A antibody
Anti-KIAA0417 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance