| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Endoplasmin (754-803 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HSP90B1 |
| Gene ID: | 7184 |
| Uniprot ID: | ENPL_HUMAN |
| Immunogen Region: | 754-803 aa |
| Specificity: | Endoplasmin Polyclonal Antibody detects endogenous levels of Endoplasmin protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human GRP94 at the amino acid range 754-803 |
| Protein Name | Endoplasmin94 Kda Glucose-Regulated ProteinGrp-94Heat Shock Protein 90 Kda Beta Member 1Heat Shock Protein Family C Member 4Tumor Rejection Antigen 1Gp96 Homolog |
| Database Links | Reactome: R-HSA-1679131Reactome: R-HSA-3000480Reactome: R-HSA-381183Reactome: R-HSA-381426Reactome: R-HSA-6785807Reactome: R-HSA-8957275 |
| Cellular Localisation | Endoplasmic Reticulum LumenSarcoplasmic Reticulum LumenMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Endoplasmin antibodyAnti-94 Kda Glucose-Regulated Protein antibodyAnti-Grp-94 antibodyAnti-Heat Shock Protein 90 Kda Beta Member 1 antibodyAnti-Heat Shock Protein Family C Member 4 antibodyAnti-Tumor Rejection Antigen 1 antibodyAnti-Gp96 Homolog antibodyAnti-HSP90B1 antibodyAnti-GRP94 antibodyAnti-HSPC4 antibodyAnti-TRA1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and HSP90B1 knockdown (KD) HeLa cells using [KD Validated] HSP90B1 Rabbit mAb (STJ11107177) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186373/408079/STJ11107177_1__83326.1744894501.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HSP90B1 knockdown (KD) HeLa cells using [KD Validated] HSP90B1 Rabbit mAb (STJ11107177) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.")
