• Immunofluorescence analysis of A-549 cells using HSD3B2 Rabbit polyclonal antibody (STJ26181). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with HSD3B2, using HSD3B2 Rabbit polyclonal antibody (STJ26181) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-HSD3B2 antibody (1-287) (STJ26181)

SKU:
STJ26181

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:10-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSD3B2
Gene ID: 3284
Uniprot ID: 3BHS2_HUMAN
Immunogen Region: 1-287
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1).
Immunogen Sequence: MGWSCLVTGAGGLLGQRIVR LLVEEKELKEIRALDKAFRP ELREEFSKLQNRTKLTVLEG DILDEPFLKRACQDVSVVIH TACIIDVFGVTHRESIMNVN VKGTQLLLEACVQASVPVFI YTSSIEVAGPNSYKEIIQNG HEEEPLENTWPTPYPYSKKL AEKAVLAANGWNLKNGDTLY TCALRPTYIYGEGGPFLSAS INEALNNNGILSSVGKFSTV NPVYVGNVAWAHILALRAL
Tissue Specificity Expressed in adrenal gland, testis and ovary.
Function 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Protein Name 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 2
3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type Ii
3-Beta-Hsd Ii
3-Beta-Hsd Adrenal And Gonadal Type Includes - 3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase
3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase
Progesterone Reductase - Steroid Delta-Isomerase
Delta-5-3-Ketosteroid Isomerase
Database Links Reactome: R-HSA-193048
Reactome: R-HSA-193993
Reactome: R-HSA-194002
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Mitochondrion Membrane
Alternative Antibody Names Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 2 antibody
Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type Ii antibody
Anti-3-Beta-Hsd Ii antibody
Anti-3-Beta-Hsd Adrenal And Gonadal Type Includes - 3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibody
Anti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibody
Anti-Progesterone Reductase - Steroid Delta-Isomerase antibody
Anti-Delta-5-3-Ketosteroid Isomerase antibody
Anti-HSD3B2 antibody
Anti-HSDB3B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance