• Western blot analysis of extracts of mouse lung, using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2min.
  • Confocal imaging of paraffin-embedded Human liver using Hemopexin (HPX) Rabbit monoclonal antibody (STJ11102432, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of extracts of Human serum, using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal imaging of paraffin-embedded Rat liver using Hemopexin (HPX) Rabbit monoclonal antibody (STJ11102432, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded rat liver using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Hemopexin (HPX) in paraffin-embedded rat liver using Hemopexin (HPX) (HPX) Rabbit monoclonal antibody (STJ11102432) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat liver using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Human serum, using Hemopexin (HPX) (HPX) Rabbit monoclonal antibody (STJ11102432) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of human liver using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse lung, using Hemopexin (HPX) (HPX) Rabbit monoclonal antibody (STJ11102432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2min.
  • Immunofluorescence analysis of mouse liver using Hemopexin (HPX) (HPX) rabbit monoclonal antibody (STJ11102432) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HPX antibody (363-462) [S2MR] (STJ11102432)

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STJ11102432

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hemopexin (363-462) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPX
Gene ID: 3263
Uniprot ID: HEMO_HUMAN
Immunogen Region: 363-462
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 363-462 of human Hemopexin (HPX) (P02790).
Immunogen Sequence: AFICPGSSRLHIMAGRRLWW LDLKSGAQATWTELPWPHEK VDGALCMEKSLGPNSCSANG PGLYLIHGPNLYCYSDVEKL NAAKALPQPQNVTSLLGCTH
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylation in the 30-40 region is minor compared to glycosylation at Thr-24 and Thr-29.
Function Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Protein Name Hemopexin
Beta-1b-Glycoprotein
Database Links Reactome: R-HSA-2168880
Cellular Localisation Secreted
Alternative Antibody Names Anti-Hemopexin antibody
Anti-Beta-1b-Glycoprotein antibody
Anti-HPX antibody

Information sourced from Uniprot.org

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