Anti-HPK1/MAP4K1 antibody (N-Term) (STJ70186)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-HPK1/MAP4K1 (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ยตg/ml.IHC: Paraffin embedded Human Thymus. Recommended concent |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP4K1 |
| Gene ID | 11184 |
| Uniprot ID | M4K1_HUMAN |
| Accession Number | NP_001036065.1; NP_009112.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | DVVDPDIFNRDPR |
| Specificity | This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1). |
Additional Info
| Post Translational Modifications | Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. Tyrosine-phosphorylated after activation of hemopoietic cells. Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome. |
| Function | Serine/threonine-protein kinase, which plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. May play a role in hematopoietic lineage decisions and growth regulation. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1Hematopoietic Progenitor KinaseMapk/Erk Kinase Kinase Kinase 1Mek Kinase Kinase 1Mekkk 1 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 antibodyAnti-Hematopoietic Progenitor Kinase antibodyAnti-Mapk/Erk Kinase Kinase Kinase 1 antibodyAnti-Mek Kinase Kinase 1 antibodyAnti-Mekkk 1 antibodyAnti-MAP4K1 antibodyAnti-HPK1 antibody |
Information sourced from Uniprot.org