| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Cow/Pig/Rabbit |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Heterogeneous nuclear ribonucleoprotein U is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: |
Affinity purification |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HNRNPU |
| Gene ID: |
3192 |
| Uniprot ID: |
HNRPU_HUMAN |
| Specificity: |
hnRNP U Monoclonal Antibody detects endogenous levels of hnRNP U protein. |
| Immunogen: |
Purified recombinant human hnRNP U protein fragments expressed in E.coli. |
| Post Translational Modifications | Cleaved at Asp-100 by CASP3 during T-cell apoptosis, resulting in a loss of DNA- and chromatin-binding activities. Extensively phosphorylated. Phosphorylated on Ser-59 by PLK1 and dephosphorylated by protein phosphatase 2A (PP2A) in mitosis. Arg-739 is dimethylated, probably to asymmetric dimethylarginine (Ref.8). Arg-733 is dimethylated, probably to asymmetric dimethylarginine. Citrullinated by PADI4. |
| Function | DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation. Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling. Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression. Participates in the circadian regulation of the core clock component BMAL1 transcription. Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner. Binds to the Xist RNA. Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA. Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA.this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi). Also negatively regulates embryonic stem cell differentiation upon LIF signaling. Required for embryonic development. Binds to brown fat long non-coding RNA 1 (Blnc1).facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis. (Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein UHnrnp UGrip120Nuclear P120 RibonucleoproteinScaffold-Attachment Factor ASaf-AP120Pp120 |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72203 |
| Cellular Localisation | NucleusNucleus MatrixChromosomeNucleus SpeckleCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentromereKinetochoreSpindleSpindle PoleMidbodyCell SurfaceCytoplasmic GranuleLocalizes At Inactive X Chromosome (Xi) RegionsLocalizes In The Nucleus During InterphaseAt MetaphaseLocalizes With Mitotic Spindle Microtubules (Mts)At AnaphaseLocalizes In The Mitotic Spindle MidzoneLocalizes In Spindle Mts Proximal To Spindle Poles In A Tpx2- And Aurka-Dependent MannerThe Ser-59 Phosphorylated Form Localizes To Centrosomes During Prophase And MetaphaseTo Mitotic Spindles In Anaphase And To The Midbody During CytokinesisColocalizes With Smarca4 In The NucleusColocalizes With Cbx5 In The NucleusColocalizes With Nr3c1 In Nuclear SpecklesLocalized In Cytoplasmic Ribonucleoprotein (Rnp) Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoprotein U antibodyAnti-Hnrnp U antibodyAnti-Grip120 antibodyAnti-Nuclear P120 Ribonucleoprotein antibodyAnti-Scaffold-Attachment Factor A antibodyAnti-Saf-A antibodyAnti-P120 antibodyAnti-Pp120 antibodyAnti-HNRNPU antibodyAnti-C1orf199 antibodyAnti-HNRPU antibodyAnti-SAFA antibodyAnti-U21.1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
