• Immunofluorescence analysis of U-2 OS cells using hnRNPM antibody (STJ29017) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using hnRNPM antibody (STJ29017) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using hnRNPM antibody (STJ29017) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using hnRNPM antibody (STJ29017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNPM antibody (STJ29017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using hnRNPM antibody (STJ29017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using hnRNPM antibody (STJ29017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using hnRNPM antibody (STJ29017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HNRNPM antibody (STJ29017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HNRNPM antibody (491-730) (STJ29017)

SKU:
STJ29017

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPM (491-730) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPM
Gene ID: 4670
Uniprot ID: HNRPM_HUMAN
Immunogen Region: 491-730
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).
Immunogen Sequence: GFGLERMAAPIDRVGQTIER MGSGVERMGPAIERMGLSME RMVPAGMGAGLERMGPVMDR MATGLERMGANNLERMGLER MGANSLERMGLERMGANSLE RMGPAMGPALGAGIERMGLA MGGGGGASFDRAIEMERGNF GGSFAGSFGGAGGHAPGVAR KACQIFVRNLPFDFTWKMLK DKFNECGHVLYADIKMENGK SKGCGVVKFESPEVAERACR MMNGMKLSGREIDVRIDRN
Post Translational Modifications Sumoylated.
Function Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Protein Name Heterogeneous Nuclear Ribonucleoprotein M
Hnrnp M
Database Links Reactome: R-HSA-6803529
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein M antibody
Anti-Hnrnp M antibody
Anti-HNRNPM antibody
Anti-HNRPM antibody
Anti-NAGR1 antibody

Information sourced from Uniprot.org

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