• Western blot analysis of extracts of various cell lines, using HNRPAB antibody (STJ119592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of U-2 OS cells using HNRPAB antibody (STJ119592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HNRNPAB antibody (1-285) (STJ119592)

SKU:
STJ119592

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRantibody (1-285) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPAB
Gene ID: 3182
Uniprot ID: ROAA_HUMAN
Immunogen Region: 1-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human HNRPAB (NP_004490.2).
Immunogen Sequence: MSEAGEEQPMETTGATENGH EAVPEGESPAGAGTGAAAGA GGATAAPPSGNQNGAEGDQI NASKNEEDAGKMFVGGLSWD TSKKDLKDYFTKFGEVVDCT IKMDPNTGRSRGFGFILFKD AASVEKVLDQKEHRLDGRVI DPKKAMAMKKDPVKKIFVGG LNPEATEEKIREYFGEFGEI EAIELPMDPKLNKRRGFVFI TFKEEEPVKKVLEKKFHTVS GSKCEIKVAQPKEVYQQQQ
Tissue Specificity Ubiquitous.
Post Translational Modifications Dimethylation at Arg-322 is probably asymmetric.
Function Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
Protein Name Heterogeneous Nuclear Ribonucleoprotein A/B
Hnrnp A/B
Apobec1-Binding Protein 1
Abbp-1
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein A/B antibody
Anti-Hnrnp A/B antibody
Anti-Apobec1-Binding Protein 1 antibody
Anti-Abbp-1 antibody
Anti-HNRNPAB antibody
Anti-ABBP1 antibody
Anti-HNRPAB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance