• Western blot analysis of various lysates using Hmox1 Rabbit polyclonal antibody (STJ11105174) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from NIH/3T3 cells using Hmox1 Rabbit polyclonal antibody (STJ11105174) at 1:1000 dilution. NIH/3T3 cells were treated by arsenite (50 Mu M) at 37 °C for 8 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.

Anti-Hmox1 antibody (1-266) (STJ11105174)

SKU:
STJ11105174

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Hmox1
Gene ID: 15368
Uniprot ID: HMOX1_MOUSE
Immunogen Region: 1-266
Specificity: Recombinant Protein corresponding to a sequence within amino acids 1-266 of mouse Hmox1 (NP_034572.1).
Immunogen Sequence: ERPQPDSMPQDLSEALKEAT KEVHIQAENAEFMKNFQKGQ VSREGFKLVMASLYHIYTAL EEEIERNKQNPVYAPLYFPE ELHRRAALEQDMAFWYGPHW QEIIPCTPATQHYVKRLHEV GRTHPELLVAHAYTRYLGDL SGGQVLKKIAQKAMALPSSG EGLAFFTFPNIDSPTKFKQL YRARMNTLEMTPEVKHRVTE EAKTAFLLNIELFEELQVML TEEHKDQSPSQMASLRQRP
Post Translational Modifications A soluble form arises by proteolytic removal of the membrane anchor.
Function Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Protein Name Heme Oxygenase 1
Ho-1
P32 Protein Cleaved Into - Heme Oxygenase 1 Soluble Form
Database Links Reactome: R-MMU-189483
Reactome: -MMU-917937
Reactome: -MMU-9609523
Reactome: -MMU-9707564
Reactome: -MMU-9707587
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Heme Oxygenase 1 antibody
Anti-Ho-1 antibody
Anti-P32 Protein Cleaved Into - Heme Oxygenase 1 Soluble Form antibody
Anti-Hmox1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance