| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:2000-1:8000IHC-P:1:50-1:100IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HMGCS2 |
| Gene ID: | 3158 |
| Uniprot ID: | HMCS2_HUMAN |
| Immunogen Region: | 269-508 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human HMGCS2 (NP_005509.1). |
| Immunogen Sequence: | CYTSYRKKIQNQWKQAGSDR PFTLDDLQYMIFHTPFCKMV QKSLARLMFNDFLSASSDTQ TSLYKGLEAFGGLKLEDTYT NKDLDKALLKASQDMFDKKT KASLYLSTHNGNMYTSSLYG CLASLLSHHSAQELAGSRIG AFSYGSGLAASFFSFRVSQD AAPGSPLDKLVSSTSDLPKR LASRKCVSPEEFTEIMNQRE QFYHKVNFSPPGDTNSLFPG TWYLERVDEQHRRKYARRP |
| Tissue Specificity | Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain. Isoform 3: Highest expression detected in heart and skeletal muscle. |
| Post Translational Modifications | Succinylated. Desuccinylated by SIRT5. Succinylation, at least at Lys-83 and Lys-310, inhibits the enzymatic activity. |
| Function | Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate. |
| Protein Name | Hydroxymethylglutaryl-Coa Synthase - MitochondrialHmg-Coa Synthase3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-77111Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Hydroxymethylglutaryl-Coa Synthase - Mitochondrial antibodyAnti-Hmg-Coa Synthase antibodyAnti-3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase antibodyAnti-HMGCS2 antibody |
Information sourced from Uniprot.org