• Western blot analysis of extracts of various cell lines, using Histone H2B antibody (STJ11103152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human cervix cancer tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human liver tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human spleen tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded mouse brain tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded rat brain tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded rat kidney tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Histone H2B antibody (47-126) [S2MR] (STJ11103152)

SKU:
STJ11103152

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2BC21
H2BC4.H2BC6.H2BC7.H2BC8.H2BC10
Gene ID: 8349
3017/8339/8343/8344/8346/8347
Uniprot ID: H2B2E_HUMAN
H2B1C_HUMAN
Immunogen Region: 47-126
Specificity: A synthetic peptide corresponding to a sequence within amino acids 47-126 of human Histone H2B (O60814).
Immunogen Sequence: KQVHPDTGISSKAMGIMNSF VNDIFERIAGEASRLAHYNK RSTITSREIQTAVRLLLPGE LAKHAVSEGTKAVTKYTSAK
Tissue Specificity
Alternative Antibody Names Anti-Histone H2b Type 2-E antibody
Anti-H2b-Clustered Histone 21 antibody
Anti-Histone H2b-Gl105 antibody
Anti-Histone H2b.q antibody
Anti-H2b/Q antibody
Anti-H2BC21 antibody
Anti-H2BFQ antibody
Anti-HIST2H2BE antibody
Anti-Histone H2b Type 1-C/E/F/G/I antibody
Anti-Histone H2b.1 A antibody
Anti-Histone H2b.a antibody
Anti-H2b/A antibody
Anti-Histone H2b.g antibody
Anti-H2b/G antibody
Anti-Histone H2b.h antibody
Anti-H2b/H antibody
Anti-Histone H2b.k antibody
Anti-H2b/K antibody
Anti-Histone H2b.l antibody
Anti-H2b/L antibody
Anti-H2BC4 antibody
Anti-H2BFL antibody
Anti-HIST1H2BC.H2BC6 antibody
Anti-H2BFH antibody
Anti-HIST1H2BE.H2BC7 antibody
Anti-H2BFG antibody
Anti-HIST1H2BF.H2BC8 antibody
Anti-H2BFA antibody
Anti-HIST1H2BG.H2BC10 antibody
Anti-H2BFK antibody
Anti-HIST1H2BI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance