• Western blot analysis of extracts of various cell lines, using Histone H2B antibody (STJ11103152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human cervix cancer tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human liver tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded human spleen tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded mouse brain tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded rat brain tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Histone H2B in paraffin-embedded rat kidney tissue using Histone H2B rabbit monoclonal antibody (STJ11103152) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Histone H2B antibody (47-126) [S2MR] (STJ11103152)

SKU:
STJ11103152

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Histone H2B (47-126) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 47-126
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 47-126 of human Histone H2B (O60814).
Immunogen Sequence: KQVHPDTGISSKAMGIMNSF VNDIFERIAGEASRLAHYNK RSTITSREIQTAVRLLLPGE LAKHAVSEGTKAVTKYTSAK
Background The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. Related pseudogenes have been identified on multiple chromosomes.

Information sourced from Uniprot.org

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