| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Histone H1 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
AE-4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2ak |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow Cytometry (1-2 µg/million cells in 0.1ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunofluorescence (0.5-1 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formal |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
H1-0 |
| Gene ID: |
3005 |
| Uniprot ID: |
H10_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with human leukemia biopsy cells. Fusion partner: NS-1. |
| Post Translational Modifications | Phosphorylated on Ser-17 in RNA edited version. ADP-ribosylated on Ser-104 in response to DNA damage. |
| Function | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division. |
| Protein Name | Histone H1.0Histone H1'Histone H1(0 Cleaved Into - Histone H1.0 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeThe Rna Edited Version Has Been Localized To Nuclear SpecklesDuring MitosisIt Appears In The Vicinity Of Condensed Chromosomes |
| Alternative Antibody Names | Anti-Histone H1.0 antibodyAnti-Histone H1' antibodyAnti-Histone H1(0 Cleaved Into - Histone H1.0 - N-Terminally Processed antibodyAnti-H1-0 antibodyAnti-H1F0 antibodyAnti-H1FV antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/99752/385108/STJ23903_1__89907.1713153959.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
