| Short Description : | Rabbit polyclonal Histone H1t (110-209) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | H1-6 |
| Gene ID: | 107970 |
| Uniprot ID: | H1T_MOUSE |
| Immunogen Region: | 110-209 |
| Immunogen Sequence: | LSKKAASGNDKGKGKKSASA KAKKMGLPRASRSPKSSKTK AVKKPKATPTKASGSGRKTK GAKGVQQRKSPAKARAANPN SGKAKMVMQKTDLRKAAGRK |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 110-209 of mouse Histone H1t (NP_034507.2). |
| Tissue Specificity | Testis-specific. |
| Post Translational Modifications | Phosphorylated in early spermatids. Citrullination at Arg-56 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. |
| Function | Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration. |
| Protein Name | Histone H1tTesticular H1 Histone |
| Database Links | |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H1t antibodyAnti-Testicular H1 Histone antibodyAnti-H1-6 antibodyAnti-H1f6 antibodyAnti-H1ft antibodyAnti-H1t antibodyAnti-Hist1h1t antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99752/385108/STJ23903_1__89907.1713153959.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
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