• Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated mouse anti His-Tag antibody (STJ11103704) at 1:20000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag monoclonal antibody (STJ11103704) at 1:20000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-His-Tag antibody [S4MM] {HRP} (STJ11103704)

SKU:
STJ11103704

£173.50 - £325.50
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Host: Mouse
Applications: WB/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MM
Conjugation: HRP
Isotype: IgG2Bk
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:10000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to His tag.
Immunogen Sequence: HHHHHH
Background Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance