| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HGS |
| Gene ID: | 9146 |
| Uniprot ID: | HGS_HUMAN |
| Immunogen Region: | 548-777 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 548-777 of human HGS (NP_004703.1). |
| Immunogen Sequence: | EQQKQTVQMRAQMPAFPLPY AQLQAMPAAGGVLYQPSGPA SFPSTFSPAGSVEGSPMHGV YMSQPAPAAGPYPSMPSTAA DPSMVSAYMYPAGATGAQAA PQAQAGPTASPAYSSYQPTP TAGYQNVASQAPQSLPAISQ PPQSSTMGYMGSQSVSMGYQ PYNMQNLMTTLPSQDASLPP QQPYIAGQQPMYQQMAPSGG PPQQQPPVAQQPQAQGPPAQ GSEAQLISFD |
| Tissue Specificity | Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated. Ubiquitinated by ITCH. |
| Function | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. |
| Protein Name | Hepatocyte Growth Factor-Regulated Tyrosine Kinase SubstrateHrsProtein Pp110 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-432720Reactome: R-HSA-5689880Reactome: R-HSA-6807004Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-9013420Reactome: R-HSA-917729Reactome: R-HSA-9635644Reactome: R-HSA-9636383Reactome: R-HSA-9706019 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane ProteinCytoplasmic SideEndosomeMultivesicular Body MembraneColocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments |
| Alternative Antibody Names | Anti-Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate antibodyAnti-Hrs antibodyAnti-Protein Pp110 antibodyAnti-HGS antibodyAnti-HRS antibody |
Information sourced from Uniprot.org
