Western blot analysis of extracts of HepG2 cells, using Hex Rabbit monoclonal antibody (STJ11103082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Hex antibody (50-150) [S2MR] (STJ11103082)

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STJ11103082
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HHEX
Gene ID: 3087
Uniprot ID: HHEX_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hex (Q03014).
Immunogen Sequence: TLPSPNSSFTSLVSPYRTPV YEPTPIHPAFSHHSAAALAA AYGPGGFGGPLYPFPRTVND YTHALLRHDPLGKPLLWSPF LQRPLHKRKGGQVRFSNDQT I
Tissue Specificity Liver and promyelocytic leukemia cell line HL-60.
Function Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. Activator of WNT-mediated transcription in conjunction with CTNNB1. Establishes anterior identity at two levels.acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. Inhibits EIF4E-mediated mRNA nuclear export. May play a role in hematopoietic differentiation.
Protein Name Hematopoietically-Expressed Homeobox Protein Hhex
Homeobox Protein Hex
Homeobox Protein Prh
Proline-Rich Homeodomain Protein
Cellular Localisation Nucleus
Nuclear Body
Cytoplasm
Alternative Antibody Names Anti-Hematopoietically-Expressed Homeobox Protein Hhex antibody
Anti-Homeobox Protein Hex antibody
Anti-Homeobox Protein Prh antibody
Anti-Proline-Rich Homeodomain Protein antibody
Anti-HHEX antibody
Anti-HEX antibody
Anti-PRH antibody
Anti-PRHX antibody

Information sourced from Uniprot.org

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