| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Probable E3 Ubiquitin-Protein Ligase Herc4 (400-700) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HERC4 |
| Gene ID: |
26091 |
| Uniprot ID: |
HERC4_HUMAN |
| Immunogen Region: |
400-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2). |
| Immunogen Sequence: |
WTVNEALIQKWLSYPSGRFP VEIANEIDGTFSSSGCLNGS FLAVSNDDHYRTGTRFSGVD MNAARLLFHKLIQPDHPQIS QQVAASLEKNLIPKLTSSLP DVEALRFYLTLPECPLMSDS NNFTTIAIPFGTALVNLEKA PLKVLENWWSVLEPPLFLKI VELFKEVVVHLLKLYKIGIP PSERRIFNSFLHTALKVLEI LHRVNEKMGQIIQYDKFYIH EVQELIDIRNDYINWVQQQ |
| Tissue Specificity | Expressed in brain and testis and detected in heart and placenta. |
| Function | Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. |
| Protein Name | Probable E3 Ubiquitin-Protein Ligase Herc4Hect Domain And Rcc1-Like Domain-Containing Protein 4Hect-Type E3 Ubiquitin Transferase Herc4 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytosolShows A Punctate Cytoplasmic Distribution |
| Alternative Antibody Names | Anti-Probable E3 Ubiquitin-Protein Ligase Herc4 antibodyAnti-Hect Domain And Rcc1-Like Domain-Containing Protein 4 antibodyAnti-Hect-Type E3 Ubiquitin Transferase Herc4 antibodyAnti-HERC4 antibodyAnti-KIAA1593 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89094/357553/STJ11100022_1__83889.1713121675.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
