Anti-HEAB antibody [R53-4N-9] (STJA0035553)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human HEAB
STJA0035553
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General Information

Short DescriptionRabbit monoclonal anti-HEAB for use in WB, IHC-P and FC in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/FC
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR53-4N-9
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IHC 1:100-1:200
FC 1:50
FormulationLiquid in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolCLP1
Gene ID10978
Uniprot IDCLP1_HUMAN
ImmunogenA synthesized peptide derived from human HEAB

Additional Info

Function Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing.this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Protein Name Polyribonucleotide 5'-Hydroxyl-Kinase Clp1
Polyadenylation Factor Clp1
Polynucleotide Kinase Clp1
Pre-Mrna Cleavage Complex Ii Protein Clp1
Database Links Reactome: R-HSA-6784531
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Polyribonucleotide 5'-Hydroxyl-Kinase Clp1 antibody
Anti-Polyadenylation Factor Clp1 antibody
Anti-Polynucleotide Kinase Clp1 antibody
Anti-Pre-Mrna Cleavage Complex Ii Protein Clp1 antibody
Anti-CLP1 antibody
Anti-HEAB antibody

Information sourced from Uniprot.org

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