• Immunofluorescence analysis of U-2 OS cells using HDGF antibody (STJ27300) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using HDGF antibody (STJ27300) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using HDGF Antibody (STJ27300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using HDGF Antibody (STJ27300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using HDGF Antibody (STJ27300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HDGF antibody (STJ27300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HDGF antibody (1-240) (STJ27300)

SKU:
STJ27300

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HDGF (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDGF
Gene ID: 3068
Uniprot ID: HDGF_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
Immunogen Sequence: MSRSNRQKEYKCGDLVFAKM KGYPHWPARIDEMPEAAVKS TANKYQVFFFGTHETAFLGP KDLFPYEESKEKFGKPNKRK GFSEGLWEIENNPTVKASGY QSSQKKSCVEEPEPEPEAAE GDGDKKGNAEGSSDEEGKLV IDEPAKEKNEKGALKRRAGD LLEDSPKRPKEAENPEGEEK EAATLEVERPLPMEVEKNST PSEPGSGRGPPQEEEEEEDE EEEATKEDAEAPGIRDHES
Tissue Specificity Ubiquitous.
Post Translational Modifications Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. Phosphorylation at Ser-165 is likely to be required for secretion.
Function Isoform 1: Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein. Isoform 2: Does not have mitogenic activity for fibroblasts. Does not bind heparin. Isoform 3: Has mitogenic activity for fibroblasts. Heparin-binding protein.
Protein Name Hepatoma-Derived Growth Factor
Hdgf
High Mobility Group Protein 1-Like 2
Hmg-1l2
Database Links Reactome: R-HSA-381038
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Secreted
Extracellular Exosome
Secreted By Exosomes And Is Located Inside The Exosome
May Also Be Secreted As Free Protein Via An As Yet Unknown Pathway
Isoform 2: Nucleus
Secreted By Exosomes And Is Located On The Outer Exosome Surface
Isoform 3: Nucleus
Alternative Antibody Names Anti-Hepatoma-Derived Growth Factor antibody
Anti-Hdgf antibody
Anti-High Mobility Group Protein 1-Like 2 antibody
Anti-Hmg-1l2 antibody
Anti-HDGF antibody
Anti-HMG1L2 antibody

Information sourced from Uniprot.org

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