• Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-OC43 Spike S1 Rabbit polyclonal antibody (STJ11103406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-HCoV-OC43 Spike S1 antibody (97-196) (STJ11103406)

SKU:
STJ11103406

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Host: Rabbit
Applications: WB
Reactivity: HCoV-OC43
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-OC43 Spike S1 (97-196) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 97-196
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 97-196 of coronavirus Spike S1 (YP_009555241.1).
Immunogen Sequence: LGYLQSFNYRIDTTATSCQL YYNLPAANVSVSRFNPSTWN KRFGFIEDSVFKPRPAGVLT NHDVVYAQHCFKAPKNFCPC KLNGSCVGSGPGKNNGIGTC
Background This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA) , an important step in translesion DNA synthesis.

Information sourced from Uniprot.org

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