• Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-229E Spike S2 Rabbit polyclonal antibody (STJ11103294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HCoV-229E Spike S2 antibody (1074-1173) (STJ11103294)

SKU:
STJ11103294

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Host: Rabbit
Applications: WB
Reactivity: HCoV-229E
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-229E Spike S2 (1074-1173) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1074-1173
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1074-1173 of coronavirus Spike S2 (NP_073551.1).
Immunogen Sequence: LENKSAELNYTVQKLQTLID NINSTLVDLKWLNRVETYIK WPWWVWLCISVVLIFVVSML LLCCCSTGCCGFFSCFASSI RGCCESTKLPYYDVEKIHIQ
Background The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5 UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

Information sourced from Uniprot.org

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