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Anti-HADHB antibody (34-270) (STJ28284)

Immunofluorescence analysis of A549 cells using HADHB Rabbit polyclonal antibody (STJ28284). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using HADHB Rabbit polyclonal antibody (STJ28284) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
Western blot analysis of various lysates, using HADHB Rabbit polyclonal antibody (STJ28284) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HADHB antibody (34-270) (STJ28284)

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STJ28284

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-HADHB (34-270) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IF/ICC 1:10-1:100
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	HADHB
Gene ID:	3032
Uniprot ID:	ECHB_HUMAN
Immunogen Region:	34-270
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-270 of human HADHB (NP_000174.1).
Immunogen Sequence:	AAPAVQTKTKKTLAKPNIRN VVVVDGVRTPFLLSGTSYKD LMPHDLARAALTGLLHRTSV PKEVVDYIIFGTVIQEVKTS NVAREAALGAGFSDKTPAHT VTMACISANQAMTTGVGLIA SGQCDVIVAGGVELMSDVPI RHSRKMRKLMLDLNKAKSMG QRLSLISKFRFNFLAPELPA VSEFSTSETMGHSADRLAAA FAVSRLEQDEYALRSHSLAK KAQDEGLLSDVVPFKVP

Function	Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.
Protein Name	Trifunctional Enzyme Subunit Beta - Mitochondrial Tp-Beta Includes - 3-Ketoacyl-Coa Thiolase Acetyl-Coa Acyltransferase Beta-Ketothiolase
Database Links	Reactome: R-HSA-1482798 Reactome: R-HSA-77285 Reactome: R-HSA-77288 Reactome: R-HSA-77305 Reactome: R-HSA-77310 Reactome: R-HSA-77346 Reactome: R-HSA-77348 Reactome: R-HSA-77350
Cellular Localisation	Mitochondrion Mitochondrion Inner Membrane Mitochondrion Outer Membrane Endoplasmic Reticulum Protein Stability And Association With Membranes Require Hadha
Alternative Antibody Names	Anti-Trifunctional Enzyme Subunit Beta - Mitochondrial antibody Anti-Tp-Beta Includes - 3-Ketoacyl-Coa Thiolase antibody Anti-Acetyl-Coa Acyltransferase antibody Anti-Beta-Ketothiolase antibody Anti-HADHB antibody Anti-MSTP029 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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