| Short Description : | Mouse monoclonal anti-H type 2 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | FC/IF/IHC |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 19-OLE |
| Isotype: | IgMk |
| Conjugation: | Unconjugated |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification: | Affinity purified from tissue culture. |
| Dilution Range: | Flow Cytometry (0.5-1.0 µg/million cells in 0.1ml). Immunofluorescence (0.5-1 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min at RT; requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at R |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with mucin isolated from a mucinous colonic adenocarcinoma.Fusion partner: SP2/0. |
| Background | 19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal) , whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. |
Information sourced from Uniprot.org

