• Anti-H type 2 antibody [19-OLE] (STJ16100156)

Anti-H type 2 antibody [19-OLE] (STJ16100156)

SKU:
STJ16100156

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Host: Mouse
Applications: FC/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-H type 2 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 19-OLE
Conjugation: Unconjugated
Isotype: IgMk
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow Cytometry (0.5-1.0 µg/million cells in 0.1ml). Immunofluorescence (0.5-1 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min at RT; requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at R
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Immunogen: A BALB/c mouse was immunized with mucin isolated from a mucinous colonic adenocarcinoma.Fusion partner: SP2/0.
Background 19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal) , whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

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