Anti-H type 2 antibody [19-OLE] (STJ16100156)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgMk
ImmunogenA BALB/c mouse was immunized with mucin isolated from a mucinous colonic adenocarcinoma.Fusion partner: SP2/0.
STJ16100156
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General Information

Short DescriptionMouse monoclonal anti-H type 2 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsFC/IF/IHC
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID19-OLE
IsotypeIgMk
ConjugationUnconjugated
ConcentrationCan be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
PurificationAffinity purified from tissue culture.
Dilution RangeFlow Cytometry (0.5-1.0 µg/million cells in 0.1ml). Immunofluorescence (0.5-1 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min at RT; requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at R
FormulationPBS with 0.02% Sodium Azide.
Storage InstructionStore for up to 1 year at 2-8°C upon receipt.

Target Information

ImmunogenA BALB/c mouse was immunized with mucin isolated from a mucinous colonic adenocarcinoma.Fusion partner: SP2/0.

Additional Info

Background 19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal) , whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

Information sourced from Uniprot.org

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