• Immunohistochemistry analysis of paraffin-embedded mouse testis using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GTPBP4 antibody (STJ23895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GTPBP4 antibody (385-634) (STJ23895)

SKU:
STJ23895

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTPBP4
Gene ID: 23560
Uniprot ID: GTPB4_HUMAN
Immunogen Region: 385-634
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 385-634 of human GTPBP4 (NP_036473.2).
Immunogen Sequence: RKRMETEESRKKRERDLELE MGDDYILDLQKYWDLMNLSE KHDKIPEIWEGHNIADYIDP AIMKKLEELEKEEELRTAAG EYDSVSESEDEEMLEIRQLA KQIREKKKLKILESKEKNTQ GPRMPRTAKKVQRTVLEKEM RSLGVDMDDKDDAHYAVQAR RSRSITRKRKREDSAPPSSV ARSGSCSRTPRDVSGLRDVK MVKKAKTMMKNAQKKMNRLG KKGEADRHVFDMKPKHLLS
Function Involved in the biogenesis of the 60S ribosomal subunit. Acts as a TP53 repressor, preventing TP53 stabilization and cell cycle arrest.
Protein Name Gtp-Binding Protein 4
Chronic Renal Failure Gene Protein
Gtp-Binding Protein Ngb
Nucleolar Gtp-Binding Protein 1
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Gtp-Binding Protein 4 antibody
Anti-Chronic Renal Failure Gene Protein antibody
Anti-Gtp-Binding Protein Ngb antibody
Anti-Nucleolar Gtp-Binding Protein 1 antibody
Anti-GTPBP4 antibody
Anti-CRFG antibody
Anti-NOG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance