• Immunofluorescence analysis of L929 cells using GSTT1 Rabbit polyclonal antibody (STJ29845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GSTT1 Rabbit polyclonal antibody (STJ29845) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat kidney using GSTT1 Rabbit polyclonal antibody (STJ29845) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GSTT1 Rabbit polyclonal antibody (STJ29845) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HepG2, using GSTT1 antibody (STJ29845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GSTT1 antibody (1-240) (STJ29845)

SKU:
STJ29845

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GSTT1 (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTT1
Gene ID: 2952
Uniprot ID: GSTT1_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of GSTT1 (NP_000844.2).
Immunogen Sequence: MGLELYLDLLSQPCRAVYIF AKKNDIPFELRIVDLIKGQH LSDAFAQVNPLKKVPALKDG DFTLTESVAILLYLTRKYKV PDYWYPQDLQARARVDEYLA WQHTTLRRSCLRALWHKVMF PVFLGEPVSPQTLAATLAEL DVTLQLLEDKFLQNKAFLTG PHISLADLVAITELMHPVGA GCQVFEGRPKLATWRQRVEA AVGEDLFQEAHEVILKAKDF PPADPTIKQKLMPWVLAMI
Tissue Specificity Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in club cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.
Protein Name Glutathione S-Transferase Theta-1
Gst Class-Theta-1
Glutathione Transferase T1-1
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Glutathione S-Transferase Theta-1 antibody
Anti-Gst Class-Theta-1 antibody
Anti-Glutathione Transferase T1-1 antibody
Anti-GSTT1 antibody

Information sourced from Uniprot.org

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