| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GSTO1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 28kDa band observed in Human Liver and in Peripheral Blood Mononucleocytes lysates (calculated MW of 27.6kDa according to NP_004823.1). Recommended concentration: 0.5-2µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GSTO1 |
| Gene ID: | 9446 |
| Uniprot ID: | GSTO1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004823.1; NP_001177931.1; NP_001177932.1 |
| Specificity: | This antibody is expected to recognize all reported isoforms (NP_004823.1; NP_001177931.1; NP_001177932.1). |
| Immunogen Sequence: | KEDPTVSALLTSEKD |
| Function | Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. |
| Protein Name | Glutathione S-Transferase Omega-1Gsto-1Glutathione S-Transferase Omega 1-1Gsto 1-1Glutathione-Dependent Dehydroascorbate ReductaseMonomethylarsonic Acid ReductaseMma(V ReductaseS-(Phenacylglutathione ReductaseSpg-R |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-156590Reactome: R-HSA-196836Reactome: R-HSA-8950505 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Glutathione S-Transferase Omega-1 antibodyAnti-Gsto-1 antibodyAnti-Glutathione S-Transferase Omega 1-1 antibodyAnti-Gsto 1-1 antibodyAnti-Glutathione-Dependent Dehydroascorbate Reductase antibodyAnti-Monomethylarsonic Acid Reductase antibodyAnti-Mma(V Reductase antibodyAnti-S-(Phenacylglutathione Reductase antibodyAnti-Spg-R antibodyAnti-GSTO1 antibodyAnti-GSTTLP28 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89705/358188/STJ11100768_1__90554.1713122502.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
