• Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-GSTK1 antibody (1-226) (STJ11104362)

SKU:
STJ11104362

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glutathione S-Transferase Kappa 1 (1-226) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTK1
Gene ID: 373156
Uniprot ID: GSTK1_HUMAN
Immunogen Region: 1-226
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).
Immunogen Sequence: MGPLPRTVELFYDVLSPYSW LGFEILCRYQNIWNINLQLR PSLITGIMKDSGNKPPGLLP RKGLYMANDLKLLRHHLQIP IHFPKDFLSVMLEKGSLSAM RFLTAVNLEHPEMLEKASRE LWMRVWSRNEDITEPQSILA AAEKAGMSAEQAQGLLEKIA TPKVKNQLKETTEAACRYGA FGLPITVAHVDGQTHMLFGS DRMELLAHLLGEKWMGPIPP AVNARL
Tissue Specificity Ubiquitous.
Function Glutathione S-transferase that catalyzes the conjugation of glutathione to exogenous and endogenous compounds. Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
Protein Name Glutathione S-Transferase Kappa 1
Gst 13-13
Gst Class-Kappa
Gstk1-1
Hgstk1
Glutathione S-Transferase Subunit 13
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Glutathione S-Transferase Kappa 1 antibody
Anti-Gst 13-13 antibody
Anti-Gst Class-Kappa antibody
Anti-Gstk1-1 antibody
Anti-Hgstk1 antibody
Anti-Glutathione S-Transferase Subunit 13 antibody
Anti-GSTK1 antibody
Anti-HDCMD47P antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance