• Immunohistochemistry analysis of paraffin-embedded mouse brain using ADRBK2 Antibody (STJ22535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spinal cord using ADRBK2 Antibody (STJ22535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ADRBK2 antibody (STJ22535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-GRK3 antibody (1-300) (STJ22535)

SKU:
STJ22535

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADRBK2 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK3
Gene ID: 157
Uniprot ID: ARBK2_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ADRBK2 (NP_005151.2).
Immunogen Sequence: MADLEAVLADVSYLMAMEKS KATPAARASKRIVLPEPSIR SVMQKYLAERNEITFDKIFN QKIGFLLFKDFCLNEINEAV PQVKFYEEIKEYEKLDNEED RLCRSRQIYDAYIMKELLSC SHPFSKQAVEHVQSHLSKKQ VTSTLFQPYIEEICESLRGD IFQKFMESDKFTRFCQWKNV ELNIHLTMNEFSVHRIIGRG GFGEVYGCRKADTGKMYAMK CLDKKRIKMKQGETLALNE
Post Translational Modifications Ubiquitinated.
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
Protein Name Beta-Adrenergic Receptor Kinase 2
Beta-Ark-2
G-Protein-Coupled Receptor Kinase 3
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-8856825
Cellular Localisation Postsynapse
Presynapse
Alternative Antibody Names Anti-Beta-Adrenergic Receptor Kinase 2 antibody
Anti-Beta-Ark-2 antibody
Anti-G-Protein-Coupled Receptor Kinase 3 antibody
Anti-GRK3 antibody
Anti-ADRBK2 antibody
Anti-BARK2 antibody

Information sourced from Uniprot.org

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