| Short Description : | Rabbit polyclonal GRK1 (1-160) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRK1 |
| Gene ID: | 6011 |
| Uniprot ID: | GRK1_HUMAN |
| Immunogen Region: | 1-160 |
| Immunogen Sequence: | MDFGSLETVVANSAFIAARG SFDGSSSQPSRDKKYLAKLK LPPLSKCESLRDSLSLEFES VCLEQPIGKKLFQQFLQSAE KHLPALELWKDIEDYDTADN DLQPQKAQTILAQYLDPQAK LFCSFLDEGIVAKFKEGPVE IQDGLFQPLLQATLAHLGQA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1). |
| Tissue Specificity | Retinal-specific. Expressed in rods and cones cells. |
| Post Translational Modifications | Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492. Phosphorylation at Ser-21 is regulated by light and activated by cAMP. Farnesylation is required for full activity. |
| Function | Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. May play a role in the maintenance of the outer nuclear layer in the retina. |
| Protein Name | Rhodopsin Kinase Grk1RkG Protein-Coupled Receptor Kinase 1 |
| Database Links | Reactome: R-HSA-2514859 |
| Cellular Localisation | MembraneLipid-AnchorCell ProjectionCiliumPhotoreceptor Outer SegmentSubcellular Location Is Not Affected By Light Or Dark Conditions |
| Alternative Antibody Names | Anti-Rhodopsin Kinase Grk1 antibodyAnti-Rk antibodyAnti-G Protein-Coupled Receptor Kinase 1 antibodyAnti-GRK1 antibodyAnti-RHOK antibody |
Information sourced from Uniprot.org
