• Western blot analysis of extracts of various cell lines, using GPRC5B antibody (STJ11104128) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPRC5B antibody (304-403) [S4128RM] (STJ11104128)

SKU:
STJ11104128

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-G-Protein Coupled Receptor Family C Group 5 Member B (304-403) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4128RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPRC5B
Gene ID: 51704
Uniprot ID: GPC5B_HUMAN
Immunogen Region: 304-403
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 304-403 of human GPRC5B (NP_057319.1).
Immunogen Sequence: TPNYFDTSQPRMRETAFEED VQLPRAYMENKAFSMDEHNA ALRTAGFPNGSLGKRPSGSL GKRPSAPFRSNVYQPTEMAV VLNGGTIPTAPPSHTGRHLW
Tissue Specificity Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal cord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach.
Function Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Protein Name G-Protein Coupled Receptor Family C Group 5 Member B
A-69g12.1
Retinoic Acid-Induced Gene 2 Protein
Raig-2
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Localized In The Plasma Membrane And Perinuclear Vesicles
Alternative Antibody Names Anti-G-Protein Coupled Receptor Family C Group 5 Member B antibody
Anti-A-69g12.1 antibody
Anti-Retinoic Acid-Induced Gene 2 Protein antibody
Anti-Raig-2 antibody
Anti-GPRC5B antibody
Anti-RAIG2 antibody

Information sourced from Uniprot.org

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