Anti-GPR4 antibody (283-362) (STJ118443)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GPR4 (283-362) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR4 |
| Gene ID | 2828 |
| Uniprot ID | GPR4_HUMAN |
| Immunogen Region | 283-362 |
| Immunogen Sequence | PILYCLVNEGARSDVAKALH NLLRFLASDKPQEMANASLT LETPLTSKRNSTAKAMTGSW AATPPSQGDQVQLKMLPPAQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 283-362 of human GPR4 (NP_005273.1). |
Additional Info
| Function | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. Activated by an optimal pH of 6.8-7.2. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. May also couple with G(q) and G(12)/G(13) G proteins. Acts as a key regulator of respiratory sensitivity to CO2/H(+) in brain retrotrapezoid nucleus neurons: acts by mediating detection of protons generated by the formation of carbonic acid in the blood, an important mechanism to impulse to breathe. Also acts as a regulator of acid secretion in the kidney collecting duct by maintaining acid-base homeostasis in the kidney. Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells, possibly through the G(12)/G(13)/Rho GTPase signaling pathway. |
| Protein Name | G-Prodeshotein Coupled Receptor 4Hgpr4G-Protein Coupled Receptor 6c.lGpr6c.l |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Prodeshotein Coupled Receptor 4 antibodyAnti-Hgpr4 antibodyAnti-G-Protein Coupled Receptor 6c.l antibodyAnti-Gpr6c.l antibodyAnti-GPR4 antibody |
Information sourced from Uniprot.org