• Western blot analysis of extracts of various cell lines, using Gephyrin antibody (STJ111307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Gephyrin antibody (STJ111307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPHN antibody (520-769) (STJ111307)

SKU:
STJ111307

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPHN (520-769) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPHN
Gene ID: 10243
Uniprot ID: GEPH_HUMAN
Immunogen Region: 520-769
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human Gephyrin (NP_065857.1).
Immunogen Sequence: TVGVTEVEVNKFPVVAVMST GNELLNPEDDLLPGKIRDSN RSTLLATIQEHGYPTINLGI VGDNPDDLLNALNEGISRAD VIITSGGVSMGEKDYLKQVL DIDLHAQIHFGRVFMKPGLP TTFATLDIDGVRKIIFALPG NPVSAVVTCNLFVVPALRKM QGILDPRPTIIKARLSCDVK LDPRPEYHRCILTWHHQEPL PWAQSTGNQMSSRLMSMRSA NGLLMLPPKTEQYVELHKG
Post Translational Modifications Palmitoylated. Palmitoylation is stimulated by GABA type A receptors activity. Palmitoylation by ZDHHC12 regulates clustering at synapses.
Function Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors. Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
Protein Name Gephyrin Includes - Molybdopterin Adenylyltransferase
Mpt Adenylyltransferase
Domain G - Molybdopterin Molybdenumtransferase
Mpt Mo-Transferase
Domain E
Database Links Reactome: R-HSA-947581
Cellular Localisation Postsynaptic Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cell Membrane
Cytoplasm
Cytosol
Cytoskeleton
Cell Projection
Dendrite
Postsynaptic Density
Cytoplasmic Face Of Glycinergic Postsynaptic Membranes
Forms Clusters At Synapses
Alternative Antibody Names Anti-Gephyrin Includes - Molybdopterin Adenylyltransferase antibody
Anti-Mpt Adenylyltransferase antibody
Anti-Domain G - Molybdopterin Molybdenumtransferase antibody
Anti-Mpt Mo-Transferase antibody
Anti-Domain E antibody
Anti-GPHN antibody
Anti-GPH antibody
Anti-KIAA1385 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance