Anti-GP91-PHOX/NOX2 antibody (Internal) (STJ73628)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-GP91-PHOX/NOX2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 75kDa band observed in lysates of cell line Daudi and in Mouse Spleen lysates (calculated MW of 65.3kDa according to Human NP_000388.2 and Mouse NP_031833.3). This band was succe |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CYBB |
| Gene ID | 1536 |
| Uniprot ID | CY24B_HUMAN |
| Accession Number | NP_000388.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | CDKQEFQDAWKLPK |
Additional Info
| Post Translational Modifications | Glycosylated. Phosphorylated on Ser and Thr residues. Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation. |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. |
| Protein Name | Cytochrome B-245 Heavy ChainCgd91-PhoxCytochrome B(558 Subunit BetaCytochrome B558 Subunit BetaHeme-Binding Membrane Glycoprotein Gp91phoxNadph Oxidase 2Neutrophil Cytochrome B 91 Kda PolypeptideSuperoxide-Generating Nadph Oxidase Heavy Chain SubunitGp91-1Gp91-PhoxP22 Phagocyte B-Cytochrome |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-6798695Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinAs Unassembled Monomer May Localize To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Cytochrome B-245 Heavy Chain antibodyAnti-Cgd91-Phox antibodyAnti-Cytochrome B(558 Subunit Beta antibodyAnti-Cytochrome B558 Subunit Beta antibodyAnti-Heme-Binding Membrane Glycoprotein Gp91phox antibodyAnti-Nadph Oxidase 2 antibodyAnti-Neutrophil Cytochrome B 91 Kda Polypeptide antibodyAnti-Superoxide-Generating Nadph Oxidase Heavy Chain Subunit antibodyAnti-Gp91-1 antibodyAnti-Gp91-Phox antibodyAnti-P22 Phagocyte B-Cytochrome antibodyAnti-CYBB antibodyAnti-NOX2 antibody |
Information sourced from Uniprot.org