• Confocal imaging of Hep G2 cells using GRASP65 Rabbit monoclonal antibody (STJ11101811, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using GRASP65 Rabbit monoclonal antibody (STJ11101811, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using GRASP65 Rabbit monoclonal antibody (STJ11101811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using GRASP65 Rabbit monoclonal antibody (STJ11101811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GORASP1 antibody (341-440) [S1MR] (STJ11101811)

SKU:
STJ11101811

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GRASP65 (341-440) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GORASP1
Gene ID: 64689
Uniprot ID: GORS1_HUMAN
Immunogen Region: 341-440
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 341-440 of human GRASP65 (Q9BQQ3).
Immunogen Sequence: SGPEDICSSSSSHERGGEAT WSGSEFEVSFLDSPGAQAQA DHLPQLTLPDSLTSAASPED GLSAELLEAQAEEEPASTEG LDTGTEAEGLDSQAQISTTE
Post Translational Modifications Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking. Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis. Myristoylated.
Function Key structural protein of the Golgi apparatus. The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon. Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks. However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.
Protein Name Golgi Reassembly-Stacking Protein 1
Golgi Peripheral Membrane Protein P65
Golgi Phosphoprotein 5
Golph5
Golgi Reassembly-Stacking Protein Of 65 Kda
Grasp65
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-204005
Reactome: R-HSA-6807878
Cellular Localisation Golgi Apparatus
Cis-Golgi Network Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Alternative Antibody Names Anti-Golgi Reassembly-Stacking Protein 1 antibody
Anti-Golgi Peripheral Membrane Protein P65 antibody
Anti-Golgi Phosphoprotein 5 antibody
Anti-Golph5 antibody
Anti-Golgi Reassembly-Stacking Protein Of 65 Kda antibody
Anti-Grasp65 antibody
Anti-GORASP1 antibody
Anti-GOLPH5 antibody
Anti-GRASP65 antibody

Information sourced from Uniprot.org

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