Anti-GNA13 antibody (STJ190857)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Guanine nucleotide-binding protein subunit alpha-13 for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNA13 |
| Gene ID | 10672 |
| Uniprot ID | GNA13_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | GNA13 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation. |
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in a process independent from Rho activation. In lymphoid follicles, transmits P2RY8- and S1PR2-dependent signals that lead to inhibition of germinal center (GC) B cell growth and migration outside the GC niche. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-13G Alpha-13G-Protein Subunit Alpha-13 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-428930Reactome: R-HSA-456926Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell MembraneLipid-AnchorMelanosomeCytoplasmNucleusIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvDetected In The Cytoplasm Of Leydig Cells And In The Seminiferous EpitheliumIncluding Differentiating Cells From The Spermatogonia To Mature Spermatozoa StagesIn Round SpermatidsAlso Present In The Nuclei |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 antibodyAnti-G Alpha-13 antibodyAnti-G-Protein Subunit Alpha-13 antibodyAnti-GNA13 antibody |
Information sourced from Uniprot.org