| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Glutamate Dehydrogenase (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GLUD1 |
| Gene ID: | 2746 |
| Uniprot ID: | DHE3_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005262.1; NP_036216.2 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2). |
| Immunogen Sequence: | ESEEQKRNRVRGILR |
| Function | Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. |
| Protein Name | Glutamate Dehydrogenase 1 - MitochondrialGdh 1 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-8964539Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionEndoplasmic ReticulumMostly Translocates Into The MitochondriaOnly A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibodyAnti-Gdh 1 antibodyAnti-GLUD1 antibodyAnti-GLUD antibody |
Information sourced from Uniprot.org
