• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g GLRX3 antibody (STJ28165). Western blot was performed from the immunoprecipitate using GLRX3 antibody (STJ28165) at a dilution of 1:1000.
  • Western blot analysis of various lysates using GLRX3 Rabbit polyclonal antibody (STJ28165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-GLRX3 antibody (1-335) (STJ28165)

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STJ28165

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLRX3 (1-335) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLRX3
Gene ID: 10539
Uniprot ID: GLRX3_HUMAN
Immunogen Region: 1-335
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GLRX3 (NP_001186797.1).
Immunogen Sequence: MAAGAAEAAVAAVEEVGSAG QFEELLRLKAKSLLVVHFWA PWAPQCAQMNEVMAELAKEL PQVSFVKLEAEGVPEVSEKY EISSVPTFLFFKNSQKIDRL DGAHAPELTKKVQRHASSGS FLPSANEHLKEDLNLRLKKL THAAPCMLFMKGTPQEPRCG FSKQMVEILHKHNIQFSSFD IFSDEEVRQGLKAYSSWPTY PQLYVSGELIGGLDIIKELE ASEELDTICPKAPKLEERL
Tissue Specificity Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
Function Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates 2Fe-2S cluster insertion into a subset of cytosolic proteins. Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
Protein Name Glutaredoxin-3
Pkc-Interacting Cousin Of Thioredoxin
Picot
Pkc-Theta-Interacting Protein
Pkcq-Interacting Protein
Thioredoxin-Like Protein 2
Database Links Reactome: R-HSA-917937
Cellular Localisation Cytoplasm
Cytosol
Cell Cortex
Myofibril
Sarcomere
Z Line
Under The Plasma Membrane
After Pma Stimulation
Glrx3 And Prkcq/Pkc-Theta Translocate To A More Extended Submembrane Area
In The Z Line
Found Associated With Csrp3
Alternative Antibody Names Anti-Glutaredoxin-3 antibody
Anti-Pkc-Interacting Cousin Of Thioredoxin antibody
Anti-Picot antibody
Anti-Pkc-Theta-Interacting Protein antibody
Anti-Pkcq-Interacting Protein antibody
Anti-Thioredoxin-Like Protein 2 antibody
Anti-GLRX3 antibody
Anti-PICOT antibody
Anti-TXNL2 antibody
Anti-HUSSY-22 antibody

Information sourced from Uniprot.org

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