• Western blot analysis of extracts of various cell lines, using GLOD4 antibody (STJ116348) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using GLOD4 Rabbit polyclonal antibody (STJ116348) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GLOD4 antibody (179-298) (STJ116348)

SKU:
STJ116348

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLOD4
Gene ID: 51031
Uniprot ID: GLOD4_HUMAN
Immunogen Region: 179-298
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 179-298 of human GLOD4 (NP_057164.3).
Immunogen Sequence: DNQCKLELQGVKGGVDHAAA FGRIAFSCPQKELPDLEDLM KRENQKILTPLVSLDTPGKA TVQVVILADPDGHEICFVGD EAFRELSKMDPEGSKLLDDA MAADKSDEWFAKHNKPKASG
Tissue Specificity Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung.
Protein Name Glyoxalase Domain-Containing Protein 4
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glyoxalase Domain-Containing Protein 4 antibody
Anti-GLOD4 antibody
Anti-C17orf25 antibody
Anti-CGI-150 antibody
Anti-My027 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance