| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GLOD4 |
| Gene ID: | 51031 |
| Uniprot ID: | GLOD4_HUMAN |
| Immunogen Region: | 179-298 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 179-298 of human GLOD4 (NP_057164.3). |
| Immunogen Sequence: | DNQCKLELQGVKGGVDHAAA FGRIAFSCPQKELPDLEDLM KRENQKILTPLVSLDTPGKA TVQVVILADPDGHEICFVGD EAFRELSKMDPEGSKLLDDA MAADKSDEWFAKHNKPKASG |
| Tissue Specificity | Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung. |
| Protein Name | Glyoxalase Domain-Containing Protein 4 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Glyoxalase Domain-Containing Protein 4 antibodyAnti-GLOD4 antibodyAnti-C17orf25 antibodyAnti-CGI-150 antibodyAnti-My027 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89663/358160/STJ11100725_1__50709.1713122462.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/92940/373680/STJ113540_1__78661.1713139974.jpg?c=1 "Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
