• Immunofluorescence analysis of L929 cells using GJA8 Rabbit polyclonal antibody (STJ11103022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GJA8 Rabbit polyclonal antibody (STJ11103022) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Raji cells, using GJA8 Rabbit polyclonal antibody (STJ11103022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GJA8 antibody (234-433) (STJ11103022)

SKU:
STJ11103022

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GJA8 (234-433) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJA8
Gene ID: 2703
Uniprot ID: CXA8_HUMAN
Immunogen Region: 234-433
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-433 of human GJA8 (NP_005258.2).
Immunogen Sequence: SALKRPVEQPLGEIPEKSLH SIAVSSIQKAKGYQLLEEEK IVSHYFPLTEVGMVETSPLP AKPFNQFEEKISTGPLGDLS RGYQETLPSYAQVGAQEVEG EGPPAEEGAEPEVGEKKEEA ERLTTEEQEKVAVPEGEKVE TPGVDKEGEKEEPQSEKVSK QGLPAEKTPSLCPELTTDDA RPLSRLSKASSRARSDDLTV
Tissue Specificity Eye lens.
Function Structural component of eye lens gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.
Protein Name Gap Junction Alpha-8 Protein
Connexin-50
Cx50
Lens Fiber Protein Mp70
Database Links Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Gap Junction Alpha-8 Protein antibody
Anti-Connexin-50 antibody
Anti-Cx50 antibody
Anti-Lens Fiber Protein Mp70 antibody
Anti-GJA8 antibody

Information sourced from Uniprot.org

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