• Western blot analysis of extracts of various cell lines, using GJA5 antibody (STJ29311) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-GJA5 antibody (229-358) (STJ29311)

SKU:
STJ29311

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GJA5 (229-358) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJA5
Gene ID: 2702
Uniprot ID: CXA5_HUMAN
Immunogen Region: 229-358
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 229-358 of human GJA5 (NP_859054.1).
Immunogen Sequence: LGWKKIRQRFVKPRQHMAKC QLSGPSVGIVQSCTPPPDFN QCLENGPGGKFFNPFSNNMA SQQNTDNLVTEQVRGQEQTP GEGFIQVRYGQKPEVPNGVS PGHRLPHGYHSDKRRLSKAS SKARSDDLSV
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Protein Name Gap Junction Alpha-5 Protein
Connexin-40
Cx40
Database Links Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Gap Junction Alpha-5 Protein antibody
Anti-Connexin-40 antibody
Anti-Cx40 antibody
Anti-GJA5 antibody

Information sourced from Uniprot.org

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